Recombinant Mouse Interleukin-6(IL-6)

Cat.No.:CYT-P08505

Background

Interleukin-6 (IL-6) encoded by the IL-6 gene, acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response. It plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It also induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. The mouse IL-6 is a single non-glycosylated polypeptide chain containing 187 amino acids and it signals through a cell-surface type I cytokine receptor complex consisting of the ligand-binding IL-6R¦Áchain (CD126), and the signal- transducing component gp130 (also called CD130). The mouse IL-6 shares 40 % and 85 % a.a. sequence identity with human and rat IL-6 and it is equally active on human and rat cells.

Synonyms£ºB-cell hybridoma growth factor, Interleukin HP-1.

Accession£ºP08505

GeneID£º16193

Source£ºEscherichia coli.

Molecular Weight£ºApproximately 21.7 kDa, a single non-glycosylated polypeptide chain containing 188 AAs.

AA Sequence£ºMFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT

Purity£º> 97 % by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of IL-6-dependent murine 7TD1 cells is less than 0.02 ng/ml, corresponding to a specific activity of > 5¡Á10⁷ IU/mg.

Physical Appearance£ºSterile Filtered White lyophilized (freeze-dried) powder.

Formulation£ºLyophilized from a 0.2 ¦Ìm filtered solution in 30 mM Acetic Acid, pH 3.0, 150 mM NaCl, 5 % Trehalose, 0.02 % Tween-20.

Endotoxin£ºLess than 1 EU/µg of rMuIL-6 as determined by LAL method.

Reconstitution£ºWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Ü -20¡æ. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70¡æas supplied.

- 1 month, 2 to 8¡æunder sterile conditions after reconstitution.

- 3 months, -20 to -70¡æunder sterile conditions after reconstitution.

 Order Information

Usage:This material is offered by InCellGen for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.